منابع مشابه
Purification of hog kidney renin with immobilized monoclonal antirenin.
Spleen cells from mice immunized with partially purified hog kidney renin were fused with mouse myeloma cells to produce a stable monoclonal hybridoma cell line that synthesizes an antibody against renin. A single monoclonal antibody was chosen for study and has been produced in large quantity and purified by affinity chromatography on protein A-Sepharose. The antirenin, which belongs to the Ig...
متن کاملLarge scale purification of hog renin. Physicochemical characterization.
Renin was purified from 47 kg of hog kidney to produce enough enzyme for enzymatic and physicochemical characterization. The procedure included extraction at pH 3.5 in the presence of protease inhibitors, two ammonium sulfate precipitations, ion exchange chromatography on Sepharose-hexamethylenediamino-pepstatin gel, gel filtration, and isoelectric focusing. Renin, 2.3 mg, with a specific activ...
متن کاملStudies on high molecular weight renin from hog kidney.
blood pressure in hypertensive rau. Clin Exp Pharmacol Physiol Suppl 2: 201-205, 1975 15. Furchgott RF, Bhadrakom S: Reactions of strips of rabbit aorta to epinephrine, isopropylarterenol, sodium nitrite and other drugs. J Pharmacol Exp Ther 108: 129-143, 1953 16. Rondell P, Gross F: Method for isometric recording from isolated vessels under various conditions. Helv Physiol Acta 18: 366-375, 19...
متن کاملSpecific antibody to hog renal renin and its application to the direct radioimmunoassay of renin in various organs.
We produced anti-hog renin antibodies using as antigens pure hog renal renin that either had been insolubilized or conjugated to tetanus toxoid. High titer antibodies were obtained, which demonstrated different cross-reactivity with renins from other species. A direct radioimmunoassay for renin was developed using antibody, monoiodinated 125I-hog renin, and various methods for separating free a...
متن کاملDeterminants of Plasma Renin Activity
The plasma renin activity (PRA) is affected by a number of environmental factors. However, significant heritability has been shown for the activity. A hypothesis that a candidate regulatory single-nucleotide polymorphism, C-5312T, of human renin gene should have a significant effect on PRA was elucidated and updating of independent determinants of PRA was attempted. Cross sectional study.
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ژورنال
عنوان ژورنال: The Journal of Japan Atherosclerosis Society
سال: 1980
ISSN: 0386-2682,2185-8284
DOI: 10.5551/jat1973.8.1_133